Reactive carbonyl species (RCSs) such as formaldehyde (FA), glyoxal (GO), methylglyoxal (MGO), and glyoxylate (GA) are reactive aldehydes, which are widely used in food preservative, paint and cosmetic industries, and medical science. For example, FA is used to preserve fruits, milk, meat, fish etc. Similarly, GO and MGO is mainly used in the process of caramelization and microbial fermentation and can also be found in dry food and beverages. These reactive species are also produced endogenously via various enzymatic and metabolic pathways in physiology. These RCSs are prone to react with multiple bio-molecules leading to advanced glycation end products (AGEs), stable mutagenic DNA adducts, DNA-protein crosslinks (DPCs). These adducts are caused by harmful effects and lead to cellular dysfunctions and disease progression. Thus, elevated levels of RCSs are associated with several diseases, such as type 2 diabetes, cardiovascular, kidney dysfunctions, chronic inflammation, and cancer. Hence, monitoring of these RCSs in an inexpensive way is very much necessary for affordable disease diagnosis.
Due to the simplicity, cost-effectiveness, ease of access, high sensitivity, fast and non-invasive fluorescence technique has gained enormous attention in the scientific communities in the last two decades. This doctoral work has reported four efficient fluorescent probes which are capable of detecting different RCSs by turn-on fluorescence response. The rational design, synthesis of activity-based fluorescence probes (ABFP), and their sensing mechanism are well explained using different spectroscopy and quantum chemical calculations. By tuning the intramolecular charge transfer (ICT) process, mono-carbonyl can be discriminated from di-carbonyls for the first time. These ABFP were also applied in food and body fluid analysis. Interestingly, GA, a biomarker of type 2 diabetes, can also be efficiently detected using developed probes that may lead to the early diagnosis of diabetes. Furthermore, fluorescence imaging of endogenous RCSs in cells can monitor cellular dysfunctions caused by FA, MGO, GO and GA and monitor the diagnosis of disease progression at the cellular level.
Ph.D. dissertation, Shiv Nadar University 2022 "Rational Design and Synthesis of Activity-Based Fluorescent Probes for Sensing of Reactive Carbonyl Species (RCSs) and their Applications "
List of publications (Included in Thesis)
1. Jana, A.; Baruah, M.; Munan, S.; Samanta, A.*; ICT based water-soluble fluorescent probe for discriminating mono and dicarbonyl species and food analysis. Chem. Commun. 2021, 57, 6380-6383.
2. Jana, A.; Joseph, M. M.; Munan, S.; Shamna, K.; Maiti, K. K.; Samanta A.*; A single benzene fluorescent probe for efficient formaldehyde sensing in living cells using glutathione as an amplifier. J. Photochem. Photobiol. B: Biol. 2021, 214, 112091.
3. Jana, A.; Joseph, M. M.; Munan, S.; Maiti, K. K.; Samanta, A.*; Highly selective chemosensor for Reactive Carbonyl Species based on simple 1,8-diaminonaphthalene. J. Photochem. Photobiol. B: Biol. 2020, 213, 112076.
4. Jana, A.$; Munan, S.$; Samanta, A.*; Activity-based fluorescent probe for sensing of formaldehyde and type 2 diabetic metabolite, glyoxylic acid in foods and body fluids (Manuscript under preparation). $ represents equal contribution.
5. Jana A., Baruah M., Samanta A.*, Activity based Fluorescent Probes for sensing and Imaging of Reactive Carbonyl Species. Chem. Asian J. 2022, e202200044.
List of publications (Not concluded in the thesis)
6. Munan, S.; Shamna, K.$; Joseph, M. M.$; Jana, A.; Ali, M.; Mapa, K.; Maiti, K. K.; Samanta, A.*; IndiFluors: A New Full-Visible Color-Tunable Donor-Acceptor–Donor (D1–A–D2) Fluorophore Family for Ratiometric pH Imaging during Mitophagy. ACS Sens. (10.1021/acssensors.1c02381). $ represents equal contribution.
7. Baruah, M.; Jana, A.; Samanta A*.; An efficient PeT based fluorescent probe for mapping mitochondrial oxidative stress produced via the Nox2 pathway. J. Mater. Chem. B, 2022,10, 2230-2237.
8. Wani, T. H.$; Surendran, S.$; Jana, A.; Chakrabarty, A.; Chowdhury, G*.; Quinone based antitumor agent sepantronium bromide (YM155) causes oxygen-independent redox-activated oxidative DNA damage. Chem. Res. Toxicol. 2018, 31(7), 612-618. $ represents equal contribution.
9. Samanta, A.*; Munan, S.; Jana, A.; Chang, T Y.*; Diversity orientated fluorescence library approach: accelerating the probe development for biological and environmental applications, Fluorescence Chemosensor, will be published by: The Royal Society of Chemistry (2022) (Book Chapter Under Review).
Research Advisor: Dr. Animesh Samanta, Assistant Professor